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KMID : 0383419690100030421
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Korean Modern Medical Journal
1969 Volume.10 No. 3 p.421 ~ p.429
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Characteristics and immunogenicity of gamma-irradiated Staphylococcus aureus
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Abstract
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Staphylococcus aureus strains 1503, 2253 and 28 were irradiated with lethal dosages of gamma ray. The characteristics in oxidative metabolism and ability of producing immunity in mice of the irradiated cells were compared with those of the vaccines made by conventional methods. The results obtained are as follows.
1. Lethal dosage of gamma irradiation to the lyophilized cells was 2ⅹ10^(5) r while being only 7ⅹ10^(5) r to the cells of suspension state.
2. Gamma-irradiated cells could respire considerably, while losing reproductive function, when given lethal dosages. The rate of oxygen uptake was noticeably high in the cells irradiated in lyophilized state, compared with that of cells irradiated in suspension state, suggesting that lyophilized state is better than the suspension state in protecting the antigenicity of the irradiated cells.
3. The lethal dose of Staphylococcus aureus strain 1503 to mice was in the neighbourhood of 5ⅹ10^(8) when injected. Intraperitoneally with 1 ml of 5% mucin. The dosage of gamma-irradiated vaccine necessary in protecting the mice from the challenge was 0.2?0.5 mg.
4. The gamma-irradiated Staphylococcus vaccine was found to be better in providing immunity than heat-treated (60℃ 10 min), phenol-treated (1%, 48hr) and formalin-treated (0.5%, 48hr) ones through a series of comparative animal inoculation tests.
5. Adjuvants, such as Freund´s and aluminum hydroxide solution, turned out not giving much effect on the immunogenicity of gamma-irradiated vaccine in mice.
6. The characteristics, prospect and possibility of application to various infectious diseases of the gamma-irradiated vaccine were throughly discussed.
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